Translational_Unit
Part:BBa_K567014:Design
Designed by: You Wang Group: iGEM11_SJTU-BioX-Shanghai (2011-09-29)
PT7-metGM
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2257
Illegal XbaI site found at 48
Illegal PstI site found at 1508 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2257
Illegal PstI site found at 1508 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2257
Illegal BamHI site found at 1951 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2257
Illegal XbaI site found at 48
Illegal PstI site found at 1508 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2257
Illegal XbaI site found at 48
Illegal PstI site found at 1508 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Overlapping PCR is used
Source
metG from E.coli. Kana gene derived from pET28a