Translational_Unit

Part:BBa_K567014:Design

Designed by: You Wang   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)


PT7-metGM


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2257
    Illegal XbaI site found at 48
    Illegal PstI site found at 1508
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2257
    Illegal PstI site found at 1508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2257
    Illegal BamHI site found at 1951
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2257
    Illegal XbaI site found at 48
    Illegal PstI site found at 1508
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2257
    Illegal XbaI site found at 48
    Illegal PstI site found at 1508
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Overlapping PCR is used


Source

metG from E.coli. Kana gene derived from pET28a

References